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 | Acceso al texto completo restringido a Biblioteca INIA Treinta y Tres. Por información adicional contacte bibliott@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Treinta y Tres. |
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Fecha : |
28/03/2022 |
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Actualizado : |
02/12/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
DE ARMAS, S.; GALVÁN, G. A.; LAPAZ, M. I.; GONZÁLEZ-BARRIOS, P.; VICENTE, E.; PIANZZOLA, M. J.; SIRI, M. I. |
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Afiliación : |
STEFANIE DE ARMAS, Área Microbiología, Departamento de Biociencias, Facultad de Química, UDELAR, Montevideo, UY.; Programa de Desarrollo de las Ciencias Básicas (PEDECIBA).; GUILLERMO A. GALVÁN, Departamento de Producción Vegetal, Centro Regional Sur (CRS), Facultad de Agronomía, UDELAR, Canelones, UY. Programa de Desarrollo de las Ciencias Básicas (PEDECIBA).; MARÍA I. LAPAZ, Área Microbiología, Departamento de Biociencias, Facultad de Química, UDELAR, Montevideo, uy. Programa de Desarrollo de las Ciencias Básicas (PEDECIBA).; PABLO GONZÁLEZ-BARRIOS, Departamento de Biometría, Estadística y Computación. Facultad de Agronomía, UDELAR, Montevideo, UY.; CARLOS ESTEBAN VICENTE CASTRO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIA J. PIANZZOLA, Área Microbiología, Departamento de Biociencias, Facultad de Química, UDELAR, Montevideo, UY. Programa de Desarrollo de las Ciencias Básicas (PEDECIBA).; MARÍA I. SIRI, Área Microbiología, Departamento de Biociencias, Facultad de Química, UDELAR, Montevideo, UY. Programa de Desarrollo de las Ciencias Básicas (PEDECIBA). |
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Título : |
Phylogeny and identification of Pantoea species associated with bulb rot and bacterial leaf blight of onion crops in Uruguay. |
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Fecha de publicación : |
2022 |
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Fuente / Imprenta : |
Plant Disease, 2022, volume 106, issue 4, pp. 1216-1225. doi: https://doi.org/10.1094/PDIS-06-21-1140-RE |
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DOI : |
10.1094/PDIS-06-21-1140-RE |
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Idioma : |
Inglés |
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Notas : |
Published Online:27 Mar 2022.
Corresponding author: María I. Siri; Email: msiri@fq.edu.uy. |
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Contenido : |
Abstract
Onion is among the most consumed vegetables in Uruguay, grown in the northwestern and southern regions of the country. The onion supply presents interannual variations associated with significant postharvest losses, mainly caused by bacterial rots. Besides bulb rotting, onion leaf lesions as well as infections on seed-stalks during seed production may be devastating for some varieties under conducive conditions. This research aimed to identify the causal agents of bulb rots and leaf blight of onion crops in Uruguay. Symptomatic bulbs, seeds-stalks, and leaves were collected from commercial fields from 2015 to 2020. Bacterial colonies were isolated and identified at genera level using physiological tests and 16S rRNA gene sequence analysis. A collection of 59 Pantoea spp. isolates was obtained (11 from bulbs and 48 from leaves and seeds-stalks). Multilocus sequence analysis using four housekeeping genes (rpoB, gyrB, leuS, and fusA) allowed the assignment of the isolates to five Pantoea species: P. ananatis, P. agglomerans, P. allii, P. eucalypti, and P. vagans. The last two species were not previously reported as onion pathogens elsewhere. The ability to cause disease symptoms was tested by leaf inoculation and red onion scale assays. P. ananatis isolates showed the highest aggressiveness in both assays. Specific isolates from P. allii (MAI 6022), P. eucalypti (MAI 6036), P. vagans (MAI 6050), and Pantoea sp. (MAI 6049) ranked second in aggressiveness on onion leaves, whereas only three isolates belonging to P. eucalypti (MAI 6036 and MAI 6058) and P. agglomerans (MAI 6045) exhibited the same scale-clearing phenotype as P. ananatis. Leaf inoculation assays were also performed on a set of eight onion cultivars and breeding lines. Overall, P. ananatis MAI 6032 showed the highest aggressiveness in all tested cultivars, followed by P. eucalypti MAI 6036. The presence of new reported bacterial species leads to complex disease management and highlights the need for further studies on virulence factors and the epidemiology of these pathogens. MenosAbstract
Onion is among the most consumed vegetables in Uruguay, grown in the northwestern and southern regions of the country. The onion supply presents interannual variations associated with significant postharvest losses, mainly caused by bacterial rots. Besides bulb rotting, onion leaf lesions as well as infections on seed-stalks during seed production may be devastating for some varieties under conducive conditions. This research aimed to identify the causal agents of bulb rots and leaf blight of onion crops in Uruguay. Symptomatic bulbs, seeds-stalks, and leaves were collected from commercial fields from 2015 to 2020. Bacterial colonies were isolated and identified at genera level using physiological tests and 16S rRNA gene sequence analysis. A collection of 59 Pantoea spp. isolates was obtained (11 from bulbs and 48 from leaves and seeds-stalks). Multilocus sequence analysis using four housekeeping genes (rpoB, gyrB, leuS, and fusA) allowed the assignment of the isolates to five Pantoea species: P. ananatis, P. agglomerans, P. allii, P. eucalypti, and P. vagans. The last two species were not previously reported as onion pathogens elsewhere. The ability to cause disease symptoms was tested by leaf inoculation and red onion scale assays. P. ananatis isolates showed the highest aggressiveness in both assays. Specific isolates from P. allii (MAI 6022), P. eucalypti (MAI 6036), P. vagans (MAI 6050), and Pantoea sp. (MAI 6049) ranked second in aggressiveness on onion leaves... Presentar Todo |
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Palabras claves : |
ALLIUM CEPA; CENTER ROT; PANTOEA SPP; PATHOGEN DIVERSITY. |
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Asunto categoría : |
-- |
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Marc : |
LEADER 03015naa a2200265 a 4500
001 1062926
005 2022-12-02
008 2022 bl uuuu u00u1 u #d
024 7 $a10.1094/PDIS-06-21-1140-RE$2DOI
100 1 $aDE ARMAS, S.
245 $aPhylogeny and identification of Pantoea species associated with bulb rot and bacterial leaf blight of onion crops in Uruguay.$h[electronic resource]
260 $c2022
500 $aPublished Online:27 Mar 2022. Corresponding author: María I. Siri; Email: msiri@fq.edu.uy.
520 $aAbstract Onion is among the most consumed vegetables in Uruguay, grown in the northwestern and southern regions of the country. The onion supply presents interannual variations associated with significant postharvest losses, mainly caused by bacterial rots. Besides bulb rotting, onion leaf lesions as well as infections on seed-stalks during seed production may be devastating for some varieties under conducive conditions. This research aimed to identify the causal agents of bulb rots and leaf blight of onion crops in Uruguay. Symptomatic bulbs, seeds-stalks, and leaves were collected from commercial fields from 2015 to 2020. Bacterial colonies were isolated and identified at genera level using physiological tests and 16S rRNA gene sequence analysis. A collection of 59 Pantoea spp. isolates was obtained (11 from bulbs and 48 from leaves and seeds-stalks). Multilocus sequence analysis using four housekeeping genes (rpoB, gyrB, leuS, and fusA) allowed the assignment of the isolates to five Pantoea species: P. ananatis, P. agglomerans, P. allii, P. eucalypti, and P. vagans. The last two species were not previously reported as onion pathogens elsewhere. The ability to cause disease symptoms was tested by leaf inoculation and red onion scale assays. P. ananatis isolates showed the highest aggressiveness in both assays. Specific isolates from P. allii (MAI 6022), P. eucalypti (MAI 6036), P. vagans (MAI 6050), and Pantoea sp. (MAI 6049) ranked second in aggressiveness on onion leaves, whereas only three isolates belonging to P. eucalypti (MAI 6036 and MAI 6058) and P. agglomerans (MAI 6045) exhibited the same scale-clearing phenotype as P. ananatis. Leaf inoculation assays were also performed on a set of eight onion cultivars and breeding lines. Overall, P. ananatis MAI 6032 showed the highest aggressiveness in all tested cultivars, followed by P. eucalypti MAI 6036. The presence of new reported bacterial species leads to complex disease management and highlights the need for further studies on virulence factors and the epidemiology of these pathogens.
653 $aALLIUM CEPA
653 $aCENTER ROT
653 $aPANTOEA SPP
653 $aPATHOGEN DIVERSITY
700 1 $aGALVÁN, G. A.
700 1 $aLAPAZ, M. I.
700 1 $aGONZÁLEZ-BARRIOS, P.
700 1 $aVICENTE, E.
700 1 $aPIANZZOLA, M. J.
700 1 $aSIRI, M. I.
773 $tPlant Disease, 2022, volume 106, issue 4, pp. 1216-1225. doi: https://doi.org/10.1094/PDIS-06-21-1140-RE
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha actual : |
01/03/2019 |
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Actualizado : |
15/01/2020 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
HAMOND, C.; SILVEIRA, C.S.; BURONI, F.; SUANES, A.; NIEVES, C.; SALABERRY, X.; ARÁOZ, V.; COSTA, R.A. DA; RIVERO, R.; GIANNITTI, F.; ZARANTONELLI, L. |
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Afiliación : |
CAMILA HAMOND, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay.//Unidad Mixta Pasteur + INIA (UMPI), Institut Pasteur de Montevideo, Uruguay.; CAROLINE DA SILVA SILVEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FLORENCIA BURONI, División Laboratorios Veterinarios Miguel C. Rubino, Laboratorio Regional Noroeste, Ministerio de Ganadería Agricultura y Pesca, Paysandú, Uruguay.; ALEJANDRA SUANES, Departamento de Bacteriología, División Laboratorios Veterinarios Miguel C. Rubino, Sede Central, Ministerio de Ganadería Agricultura y Pesca, Montevideo, Uruguay.; CECILIA NIEVES, Unidad Mixta Pasteur + INIA (UMPI), Institut Pasteur de Montevideo, Uruguay.//Laboratorio de Microbiología Molecular & Estructural, Institut Pasteur de Montevideo, Montevideo, UruguaY.; XIMENA SALABERRY, Departamento de Bacteriología, División Laboratorios Veterinarios Miguel C. Rubino, Sede Central, Ministerio de Ganadería Agricultura y Pesca, Montevideo, Uruguay.; VIRGINIA ARÁOZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RICARDO ALMEIDA DA COSTA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RODOLFO RIVERO, División Laboratorios Veterinarios Miguel C. Rubino, Laboratorio Regional Noroeste, Ministerio de Ganadería Agricultura y Pesca, Paysandú, Uruguay.; FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay.//6Veterinary Population Medicine Department, College of Veterinary Medicine, University of Minnesota, Saint Paul, MN, USA.; Unidad Mixta Pasteur + INIA (UMPI), Institut Pasteur de Montevideo, Montevideo, Uruguay./Laboratorio de Microbiología Molecular & Estructural, Institut Pasteur de Montevideo, Montevideo, Uruguay. |
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Título : |
Leptospira interrogans serogroup Pomona serovar Kennewicki infection in two sheep flocks with acute leptospirosis in Uruguay. |
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Fecha de publicación : |
2019 |
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Fuente / Imprenta : |
Transboundary and Emerging Diseases ,2019 May, Vol. 66 (3), p. 1186-1194. DOI: https://doi.org/10.1111/tbed.13133 |
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DOI : |
10.1111/tbed.13133 |
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Idioma : |
Inglés |
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Notas : |
Article history: First published: 26 January 2019 /Received: 17 August 2018 / Revised: 18 January 2019 / Accepted: 19 January 2019. |
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Contenido : |
Abstract:Acute leptospirosis is an infrequent disease in sheep that can cause jaundice, haemolysis, haemoglobinuria, hepatitis and nephritis. In most reports the diagnoses have been made by clinical, pathological or serological evidence without isolation or direct identi?cation of the agent. Here, we report one con?rmed and one presumptive outbreak of acute leptospirosis in suckling lambs from two unrelated sheep farms in Uruguay with mortalities of 9/60 (15%) and 9/163 (5.5%) lambs. Both outbreaks occurred in Sep?Oct 2017 after heavy rainfall and ?ooding events. The main gross and histologic pathological ?ndings in two autopsied lambs, one from each farm, included severe di?use jaundice, haemoglobinuria, acute necrotizing hepatitis with cholestasis and interstitial nephritis. Leptospira interrogans serogroup Pomona serovar Kennewicki was isolated from sheep in both ?ocks and the same genotype was identi?ed directly in clinical samples from infected animals, including one of the deceased lambs subjected to autopsy, by ampli?cation and partial sequencing of rrs and secY genes. This serovar has recently been identi?ed in infected cattle and humans in Uruguay . The impact of Leptospira spp. infection in ovine health, and the epidemiologic role of sheep as reservoirs of leptospirosis for humans and animals need further investigation. © 2019 Blackwell Verlag GmbH |
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Palabras claves : |
ACUTE LEPTOSPIROSIS; ENFERMEDADES DE LOS RUMIANTES; MOLECULAR DIAGNOSTICS; MORTALITY; PLATAFORMA DE SALUD ANIMAL; SALUD ANIMAL; SHEEP. |
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Thesagro : |
OVEJA; URUGUAY. |
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Asunto categoría : |
L73 Enfermedades de los animales |
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Marc : |
LEADER 02629naa a2200373 a 4500
001 1059551
005 2020-01-15
008 2019 bl uuuu u00u1 u #d
024 7 $a10.1111/tbed.13133$2DOI
100 1 $aHAMOND, C.
245 $aLeptospira interrogans serogroup Pomona serovar Kennewicki infection in two sheep flocks with acute leptospirosis in Uruguay.$h[electronic resource]
260 $c2019
500 $aArticle history: First published: 26 January 2019 /Received: 17 August 2018 / Revised: 18 January 2019 / Accepted: 19 January 2019.
520 $aAbstract:Acute leptospirosis is an infrequent disease in sheep that can cause jaundice, haemolysis, haemoglobinuria, hepatitis and nephritis. In most reports the diagnoses have been made by clinical, pathological or serological evidence without isolation or direct identi?cation of the agent. Here, we report one con?rmed and one presumptive outbreak of acute leptospirosis in suckling lambs from two unrelated sheep farms in Uruguay with mortalities of 9/60 (15%) and 9/163 (5.5%) lambs. Both outbreaks occurred in Sep?Oct 2017 after heavy rainfall and ?ooding events. The main gross and histologic pathological ?ndings in two autopsied lambs, one from each farm, included severe di?use jaundice, haemoglobinuria, acute necrotizing hepatitis with cholestasis and interstitial nephritis. Leptospira interrogans serogroup Pomona serovar Kennewicki was isolated from sheep in both ?ocks and the same genotype was identi?ed directly in clinical samples from infected animals, including one of the deceased lambs subjected to autopsy, by ampli?cation and partial sequencing of rrs and secY genes. This serovar has recently been identi?ed in infected cattle and humans in Uruguay . The impact of Leptospira spp. infection in ovine health, and the epidemiologic role of sheep as reservoirs of leptospirosis for humans and animals need further investigation. © 2019 Blackwell Verlag GmbH
650 $aOVEJA
650 $aURUGUAY
653 $aACUTE LEPTOSPIROSIS
653 $aENFERMEDADES DE LOS RUMIANTES
653 $aMOLECULAR DIAGNOSTICS
653 $aMORTALITY
653 $aPLATAFORMA DE SALUD ANIMAL
653 $aSALUD ANIMAL
653 $aSHEEP
700 1 $aSILVEIRA, C.S.
700 1 $aBURONI, F.
700 1 $aSUANES, A.
700 1 $aNIEVES, C.
700 1 $aSALABERRY, X.
700 1 $aARÁOZ, V.
700 1 $aCOSTA, R.A. DA
700 1 $aRIVERO, R.
700 1 $aGIANNITTI, F.
700 1 $aZARANTONELLI, L.
773 $tTransboundary and Emerging Diseases ,2019 May, Vol. 66 (3), p. 1186-1194. DOI: https://doi.org/10.1111/tbed.13133
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